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Figure 3


Fig. 3 . Expression of Hoxd and flanking genes during ES cell differentiation. RT-PCR analysis of Hoxd and flanking genes (Mtx2, Evx2 and Lnp) in undifferentiated (Un) OS25 ES cells and during 18 days of RA-induced differentiation. Loss of Oct4 expression was used to monitor differentiation and Hprt serves as a constitutively expressed control. +, with RT; -, without RT.