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Figure 4


Fig. 4. Arhgef11 is not required for formation of Kupffer's vesicle and cilia. (A,B) Posterior region of live, uninjected WT and MO-injected embryos. The inset is a magnified view of Kupffer's vesicle. White scale bar, 100 µm; black scale bar, 25 µm. (C,D) Acetylated tubulin immunostaining of cilia within Kupffer's vesicle. Scale bar, 10 µm. (E) Comparison of Kupffer's vesicle cilia length for WT control, MO-injected (AUG, SPL) and vu7/vu7 embryos. The asterisk marks significantly different lengths (P≤0.001).