Fig. 4. Mouse, but not human, ZP2 is cleaved following fertilization. Immunoblot of eggs (lanes 1,3,5) and two-cell embryos (lanes 2,4,6) from wild-type (referred to as `normal') (lanes 1 and 2), huZP2 rescue (lanes 3 and 4) and huZP2 transgenic (lanes 5-6) female mice. After SDS-PAGE run under reducing conditions (5% ß-mercaptoethanol), immunoblots were incubated with monoclonal antibodies specific to the carboxyl fragment of mouse (faux colored green) or human (faux colored red) ZP2 and visualized with a secondary antibody and chemiluminescence. Molecular masses (kDa) are indicated on the left.