Fig. 6. The cellular localization of Fxna. (A) Predicted cellular localization domains of Fxna. The graph depicts the probability of different regions of the Fxna protein being located on the outer or inner side of a cellular membrane, or embedded within the membrane. Red, membrane-associated domains; green line, outer side of membrane; blue line, inner side of membrane. The catalytic domain is located on the outer side of a cellular membrane between amino acid residues 163-393. (B-D) Double immunohistofluorescence images showing the colocalization of Fxna-Flag (green) with PDI, an ER-specific marker (red). COS-7 cells were transfected with Fxna-Flag 24 hours prior to staining with a monoclonal antibody against Flag and a polyclonal antibody against PDI. Note that non-transfected cells are only positive for PDI. Cell nuclei are stained with Hoescht 33258 (blue). Scale bar: 20 µm.