Fig. 6. Protein interaction network of ARP2-ARP3 complex and regulatory components. The subunits of the ARP2-ARP3 complex and upstream regulatory components have been tested for direct interactions using the yeast two-hybrid system (black lines) and Bimolecular Fluorescence Complementation (BiFC, yellow lines). In the yeast two-hybrid system, interactions were detected by growth of yeast cells on triple dropout media containing 3-20 mM 3-AT.