Fig. 2. Vari and its homologs define a new subgroup of epithelial MAGUKs. (A) A dendrogram showing the sequence similarities between Vari and other MAGUKs. Conserved subgroups are delineated by colored lines with subgroup names or members and representative domain structures adjacent. Colored lines show species representation. PALS2 is the mouse homolog of VAM-1 (Kamberov et al., 2000). Semitransparent lines beside the P55 (MPP1) subgroup indicate that while invertebrates lack distinct P55 genes, alternative splice forms of the LIN-2/Caki subgroup may be the functional equivalent.?, MAGUKs that cannot be assigned to conserved subgroups. Numbers at dendrogram node points show the percentage of dendrograms created in 1000 bootstrap iterations containing the displayed grouping. Human Carma3 was used as an outgroup to root the displayed dendrogram. (B) C-terminal alignments of the MAGUK subgroups. Residues conserved between more than 75% of shown subgroup members are marked in red. Potential PDZ-binding motifs for each subgroup are underlined in the consensus. , Dr_ZO-1 C-terminal sequence predicted from genomic sequence and is absent in current gene annotations; ^***, end of coding sequence.