Fig. 4. Loss of miR164 miRNA-mediated regulation quantitatively affects target gene expression. (A-C) Bar charts showing the results of relative qRT-PCR experiments, in which the target transcript abundance was assessed for different tissue types (as indicated) of wild-type and mir164abc triple-mutant plants. Results were normalized using TUB4 transcript levels. The transcript abundance of all predicted miR164 targets is higher in the mir164abc triple mutant (m) as compared with the wild type (wt) in all tissues tested. Fold-change differences in transcript levels between the wild type and the mir164abc triple mutant are shown. Bars represent the s.e. of the measurements.