Fig. 5. Effect of miR164 miRNA regulation on target gene expression. Representative confocal images of inflorescences of primary transformants. FM4-64 dye was used to stain plasma membranes (red). (A-D) Effects of miR164-mediated regulation on CUC1 and CUC2 expression. Consequences of permitted (A,B) and abolished (C,D) miR164-mediated regulation for translational fusions of CUC1 (A,C) and CUC2 (B,D) to GFP. The same confocal microscopy settings have been used for the images shown. Arrowheads (A,B) mark cells that weakly express GFP in boundaries between the inflorescence meristem and flower primordia. (E,F) Transcriptional reporters for CUC1 (E) and CUC2 (F) expressing the GFP variant 3xVENUS-N7 (green). Number of plants showing depicted expression pattern (x) with respect to total sample size (n_tot), indicated as ratio (x/n_tot), was 7/9 for pCUC1::CUC1-GFP (A), 6/6 for pCUC1::CUC1^m-GFP (C), 10^*/20 (^*no expression detected in others) for pCUC2::CUC2-GFP (B), 4/4 for pCUC2::CUC2^m-GFP (D), 7/8 for pCUC1::3xVENUS-N7 (E) and 6/7 for pCUC2::3xVENUS-N7 (F). Scale bars: 100 µm.