Fig. 6. Target mRNA accumulation in mir164abc triple mutants. (A-F) In situ localization of CUC2 (A,B) and CUC1 (C-F) transcripts in transverse sections of stage 9 flowers (A,B) and of inflorescences (C-F). Tissue of wild-type (A,C,E) and mir164abc mutant (B,D,F) plants was processed equivalently and was present on the same microscope slide (A,B), or was hybridized in the same slide sandwich (C,D), to allow a direct comparison of the signals obtained. Arrows (A,B) point to regions of elevated CUC2 expression in partially fused carpels (ca) of mir164abc mutants as compared with the wild type. By contrast, CUC2 expression in stamens (st) appeared to be unaffected. (C,D and their enlargements E,F) Randomly located foci of high CUC1 expression were sometimes observed within primordia (asterisks) and between primordia (arrowheads) of mir164abc mutant plants. Scale bars: 20 µm.