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Figure 2


Fig. 2. WMISH showing the dynamic pattern of Lvsnail mRNA expression during sea urchin development. (A) No staining is detected at the 16-cell stage. (B) At late-hatched blastula, Lvsnail staining appears in the vegetal region. (C) In a mesenchyme blastula, undergoing ingression, Lvsnail mRNA is detected in the ingressing PMCs (arrow). (D) In early gastrula, Lvsnail mRNA expression disappears from PMCs (arrow), and is expressed instead in SMCs. (E) Late gastrula, Lvsnail mRNA continues to be expressed in SMCs. (F,G) Lateral and vegetal (vv) views, respectively, of Prism stage embryos, showing Lvsnail mRNA in ventrolateral PMC clusters (arrow in F). (H) Early pluteus. Lvsnail mRNA is detected in the PMCs at the tips of the larval arms (arrow).