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Figure 5


Fig. 5. Effects of SnaMASO on PMC specification and differentiation. (A-D) In situ hybridization with Lvalx1 and Lvets1 probes. Control mesenchyme blastula embryos show strong expression of Lvalx1 and Lvets1 in PMCs (A,C). Expression of alx1 and ets1 are not affected by SnaMASO injection (B,D). (E,F) Immunostaining with mAb 1d5, shows the presence of 1d5 in control PMCs (E), but little to no expression of 1d5 in Sna morphants (F). (G) QPCR analysis of the expression of PMC differentiation genes, Lvsm30, Lvsm50 and Lvmsp130, in Sna morphants at the HB and MB stage relative to controls. Data are shown as net {Delta}CT ± s.e.m.