Fig. 4. Pigment patterns at early metamorphosis and after late metamorphosis. (A) Wild-type D. rerio with initially dispersed metamorphic melanophores as well as nascent stripes bounding the primary interstripe (arrow). (B) Wild-type D. albolineatus has dispersed melanophores, which are largely absent from a corresponding interstripe region (arrow). (C,D) kit mutants of both species lack melanophores at early metamorphosis. (E) Wild-type D. rerio with a juvenile pigment pattern of two primary stripes bounding the primary interstripe and a third stripe developing further ventrally. (F) Wild-type D. albolineatus with melanophores relatively uniformly dispersed except for the interstripe region. Melanophores adjacent to the interstripe tend to be larger and darker. White bar indicates darkness owing to internal structures (aorta, vertebrae, etc.) rather than melanophores. (G) kit mutant D. rerio develop residual LM melanophores in stripes (large arrowheads) dorsal and ventral to the interstripe. (H) kit mutant D. albolineatus develop residual LM melanophores primarily in a stripe dorsal to the interstripe (large arrowheads), although a few melanophores are also found ventral to the interstripe (small arrowheads). Ages, A and C, 31 dpf; B and D, 28 dpf; E and G, 53 dpf; F and H, 60 dpf. Scale bars: in A, 500 µm for A-D; in E, 500 µm for E,G; in F, 500 µm for F,H.