Fig. 5. Two MSA elements are required for sufficient expression of the KN gene. (A) The KN gene contains three MSA motifs in different orientations in its promoter region (arrowheads). The promoter-deletion construct KNMSA1 only contains a promoter fragment from -286 bp to the KN start codon (ATG, +1). In the KNMSA2 construct, two MSA motifs and the sequence between them (-278 to -250 bp) were replaced by a 12-bp sequence containing XbaI and SmaI restriction sites. (B) Western blots of Myc-tagged KN from transgenic kn mutant plants carrying KNMSA1. (C) Western blots of Myc-tagged KN from transgenic plants carrying Myc-KN rescue construct and KNMSA2. In all three independent KNMSA2 transgenic plants, only endogenous KN without the Myc-tag was detected. KN, anti-KN antiserum; Myc, peroxidase-coupled anti-Myc monoclonal antibody.