Fig. 9. Notch signaling acts in a short time-window early in pronephric
development. shippo1-positive multiciliated cells were counted at
34 hpf (in situ hybridization) in individual tubules. (A)
Quantification of MCCs per pronephric tubule in experiments adding DAPT at
progressively later times. (B) Quantification of MCCs per pronephric
tubule in embryos induced to express Notch1a ICD by heat shock at 8 hpf and 9
hpf. See text for details.