Fig. 1. Zbtb20 misexpression in dorsal telencephalon of transgenic mice. (A) Schematic illustration of the Zbtb20^S and Zbtb20^L recombinant constructs used for pronuclear microinjection. (B) Western blot analysis of Zbtb20^S and Zbtb20^L expression in the hippocampal formation of wild-type mice and mice harboring D6/Zbtb20^S (Tg^S), D6/Zbtb20^L (Tg^L) and D6/Zbtb20^S//D6/Zbtb20^L (Tg^S/L) transgenes (left). Western blot analysis of postnatal Zbtb20 expression in the hippocampal formation of Tg^S and wild-type littermates (right). (C,D,F,G) Indirect immunofluorescent detection in wild-type and Tg^S E16 and E18 coronal hemisections of Zbtb20 protein, counterstained with DAPI. (E,H) Horizontal sections of P5 wild-type and Tg^S hippocampal formations showing co-expression of Zbtb20 (red) and NeuN (green) by indirect immunofluorescence. CA1 and CA3 indicate hippocampus; CP, cortical plate; DG, dentate gyrus; Hip, presumptive hippocampus; LV, lateral ventricle; Sub, subiculum; WT, wild type.