Fig. 2. Delayed radial migration of immature projection neurons in Zbtb20 transgenic mice. (A,G) Nissl-stained wild-type and Zbtb20 transgenic E18 coronal sections of medial-dorsal telencephalon. Compared with wild type (A), Zbtb20 transgenic embryos harbor streams of radial migrating cells in the intermediate zone (arrow in G). (B-F,H-L) Cell birth date and cortical migration analyses by indirect immunofluorescent detection of BrdU-labeled cells (arrows in B,C,H,I) and by fluorescent visualization of EGFP-labeled neurons in CP (arrows in D-F,J-L) at late P0 (D,E,J,K) and P14 (F,L) following in vivo transfection with a pCIG2-EGFP expression vector at E15, in coronal sections counterstained with DAPI. (M) Distribution of EGFP-labeled cells in P0 brains transfected with pCIG2-EGFP at E15.The data are presented as means±s.e.m. ^*, P=0.021, ^**, P=0.029, ^***, P0.001. Deep, deep half of CP; Superficial, superficial half of CP.