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Figure 1


Fig. 1. ATML1 promoter activity in wild-type and mutant embryos. (A, B) ATML1 promoter-GFP reporter fusion constructs pATML1::NLS:3xEGFP (A) and pATML1::CYCB1-N:NLS:3xEGFP (B). NLS, SV40 nuclear localization signal sequence; GFP, enhanced green fluorescence protein sequence; nost, nopaline synthase terminator; CYCB1-N, a sequence encoding an N-terminal fragment of CYCLINB1;2, including the destruction box. (C-H) pATML1::NLS:3xEGFP expression in wild-type embryos at successively older stages: (C) one-cell; (D) 16-cell; (E) 32-cell; (F) mid-heart (arrowheads indicate quiescent center cells lacking GFP signal); (G) torpedo; (H) bent-cotyledon. (I,J) pATML1::CYCB1-N:NLS:3xEGFP expression in wild-type embryos at different stages: (I) two-cell (arrowhead indicates GFP expression in nucleus of suspensor cell), and (J) 16-cell (compare with D). (K-N) pATML1::NLS:3xEGFP expression in mutant embryos: (K) ball-shaped gn; (L) heart-stage mp; (M) basal peg of mp; (N) atml1;pdf2. Green, GFP signals; red, chlorophyll autofluorescence. Scale bars: 10 µm in C-G,I-N; 50 µm in H.