Fig. 5. GFP expression driven by six tandem repeats of the 179 bp
promoter fragment. The diagrams to the left indicate the position of the
179 bp fragment in the ATML1 promoter, the WUS-binding site (red
circle) and the L1 box (yellow circle). The arrow indicates the transcription
start site. Red crosses indicate mutations in cis-regulatory sequences. All
fragments consisted of six tandem copies (x 6) fused to the 35S minimal
promoter (35Smini). (A-E) 6x179bp expression at one-cell (A),
eight-cell (B), 32-cell (C), early-heart (D) and late-heart (E) stages.
(F-J) 6x179bpmL1 expression at one-cell (F), eight-cell (G),
late-globular (H), early-heart (I) and late-heart (J) stages. (K-O)
6x179bpmWUS expression at one-cell (K), eight-cell (L), 32-cell (M),
early-heart (N) and late-heart (O) stages. Scale bars: 10 µm.