Fig. 6. GFP expression driven by four overlapping subfragments of the 179 bp promoter fragment. The constructs are shown to the left, the corresponding GFP expression patterns in embryos to the right. All the promoter subfragments were hexamerized (x 6) and fused to the 35S minimal promoter (35Smini). Sequence numbering refers to the distance in bp from the transcription start site (+1; arrow in top diagram). Circles indicate the WUS-binding site (red) and L1 box (yellow). GFP expression in (A-E) 6x89bp, (F-J) 6x101bp, (K-O) 6x114bp, (P-T) 6x90bp. Embryo stages: (A,F,K,P) one-cell; (B,G,L,Q) eight-cell; (C,H,M,R) 32-cell; (D,I,N,S) mid-heart; (E,J,O,T) late-heart. White arrowheads (D,E,N) indicate reduced expression in the presumptive SAM region; yellow arrowheads (C-E,M) indicate reduced expression at the basal pole; white arrows (S) indicate patchy expression in epidermis. Scale bars: 10 µm.