Fig. 1. Expression pattern of Ptf1a protein during mouse retinogenesis. Ptf1a protein was visualized by immunostaining with an anti-Ptf1a antibody of wild-type retina at E12.5 (A,D), E16.5 (B,E) and P1 (C,F). The boxed areas in A,B,C are shown at higher magnifications in D,E,F, respectively. The expression of Ptf1a protein is first seen at E12.5 in cells scattered in the NBL (D, arrows). As development proceeds, the number of Ptf1a-expressing cells increases and some cells reach out to the innermost zone of the NBL (E,F). Scale bars: 100 µm in A-C; 50 µm in D-F. GCL, ganglion cell layer; NBL, neuroblastic layer.