Development -- Nakhai et al. 134 (6): 1151 Figure IG4

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Fig. 4. Effect of Ptf1a absence on generation of retinal cell types at E18.5. Retinal sections were prepared from Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ R26R and control Ptf1a^+/Cre⁽^ex1⁾ R26R embryos at E18.5. Co-immunostaining for Pax6 (green, A) and ß-galactosidase (red, B) shows the expression of both genes in the inner NBL of Ptf1a^+/Cre⁽^ex1⁾ R26R retina (C, inset). In Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ R26R retina, Pax6 is expressed at a low level in the NBL (D), whereas ß-galactosidase-positive cells are in the GCL (E). The merged image shows no coexpression of Pax6 and ß-galactosidase (F). Immunohistochemistry on cryosections from Ptf1a^+/Cre⁽^ex1⁾ R26R retina shows the expression of Hu/D antigen in the GCL (green, G) and ß-galactosidase in the inner NBL (red, H). ß-galactosidase-positive cells do not coexpress Hu/D (I, inset). In Ptf1a^Cre⁽^ex1⁾^/Cre⁽^ex1⁾ R26R retina, both Hu/D (J) and ß-galactosidase (K) are expressed in the GCL. Merged image shows the coexpression of Hu/D and ß-galactosidase in the GCL (L, inset, arrows). Insets in C,I and L represent a 2.67x enlargement. Background was stained by DAPI (blue). Scale bars: 50 µm. GCL, ganglion cell layer; NBL, neuroblastic layer.