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Figure 4


Fig. 4. cnox-2 acts as an upstream regulator of the nematocyte and neuronal differentiation pathways. (A) Colocalization of the cnox-2 (green) and hyZic (red) transcripts in nematoblast clusters (a-c) and dividing i-cells (e-g). After 9x cnox-2 dsRNA exposures, expression of cnox-2 and hyZic is either abolished (i-k, hydra1) or residual (m-o, hydra2). In cnox-2(-) hydra1, Kazal1 expression in gland cells (purple, l,p) is similar to control (d,h). Scale bars: 30 µm in a-d,i-o; 5 µm in e-h,p. (B) Expression of genes expressed in the nematocyte (left) and/or neuronal (right) cell lineages of hydra exposed 5x to control (lanes 1,3,5,7) or cnox-2 (lanes 2,4,6,8) dsRNAs. Number of cycles: 18 except hyZic (24), msh (16), hyCOUP-TF (16). (C) Scheme depicting the putative regulators of interstitial stem cells, neuronal progenitors, interstitial cells, neurons and nematoblasts. ic, interstitial cell; ISC, interstitial stem cell; nb, nematoblast; ne, neuron; NPG, neuronal progenitor.