Fig. 2. Dual immunofluorescence deconvolution microscopy for SMO and PTCH1 in wild-type and MMTV-SmoM2 transgenic mammary glands. Expression of SMO (A,D), PTCH1 (B,E) and merged three-color images including the DAPI counterstain (C,F) for wild-type (A-C) and MMTV-SmoM2 transgenic (D-F) mammary glands. (A) No detectable SMO expression. (B) Near uniform PTCH1 expression. (C) Merge of A and B. (D) Area of duct in a transgenic gland showing ectopic SMO expression. (E) PTCH1 expression in the same duct showing cell to cell variation in PTCH1 staining. (F) Merge of D and E showing (arrowed) SMO⁺ cells in which PTCH1 is moderately elevated relative to SMO^- cells. Scale bar: 50 µm.