Fig. 7. Dual immunofluorescence analysis of PTCH1-positive and SMO-positive human DCIS and IBC. (A-F) PTCH1-Ki67 in DCIS and IBC. Representative staining patterns are shown that indicate extensive colocalization of PTCH1 with Ki67 in those samples expressing detectable PTCH1. (G-J) SMO-Ki67 in DCIS and IBC. Representative staining patterns are shown that indicate rare colocalization of SMO with Ki67 in those samples expressing detectable SMO. Arrows and arrowheads in G indicate SMO⁺ cells and Ki67⁺ cells, respectively. (K) Quantitative analysis of SMO-Ki67 colocalization in DCIS and IBC.