Fig. 1. Tsp distribution changes following the formation of the myotendinous junction. Wild-type embryos at stage 12-13 (A-D) or stage 16 (E-H) labeled for Thrombospondin (A,E, Tsp, red), Stripe (B,F, marking all tendon cells, blue) and Myosin heavy chain (C,G, MHC, marking all somatic muscles, green) are shown, together with their merged images (D,H). Arrowheads mark the distribution of Tsp prior to muscle-tendon binding, whereas arrows mark the accumulation of Tsp at the myotendinous junction following muscle binding.