Fig. 2. Tsp levels are reduced in myospheroid mutant and are induced by Stripe. (A-D) Mutant embryos lacking functional ßPS (mys^XG43) and labeled with Tsp (A, red), Stripe (B, blue) and Myosin heavy chain (C, MHC, green) are shown. The merged image is shown in D. Arrows indicate sites where muscles are still associated with tendon cells, and Tsp is higher. Arrowheads indicate sites where muscles have detached, and Tsp is significantly low. (E,F) stripe mutant embryos stained for Tsp (E, green) and MHC (red). F is the merged image. Tsp is still detected in these embryos. (G-I) Embryos overexpressing Stripe using the engrailed-gal4 driver, stained for Tsp (G, red) and anti-Stripe (H, green). Their merged image is shown in I. Tsp is highly induced by Stripe.