Fig. 3. The CTD is deleted in tsp^8R mutation. Upper panel: a scheme depicting the genomic structure of the tsp gene (upper draw) and the location of the EP element as well as the deletion obtained by its excision (lower draw). A domain map of the TSP protein as well as the truncated protein obtained after the imprecise excision lacking the conserved CTD, Tsp repeats and four out of six EGF domains is shown. Lower panel: the merged image of wild-type embryo (A), embryo homozygous for Df(2L)BSC9, which deletes tsp locus (B), and embryo homozygous for the P-excision tsp^8R (C) stained for Tsp (red) and Myosin heavy chain (MHC) (green). Note the loss of Tsp staining in Df(2L)BSC9 and in tsp^8R mutant embryos.