Fig. 5. Muscle rounding and aggregation are observed in tsp^8R mutant embryos at stage 16. Wild-type (A-C) or tsp^8R mutant (D-F) embryos at stage 16 stained for Stripe (A,D, red) and Myosin heavy chain (B,E, MHC, green) and their corresponding merged images (C,F). The somatic muscles round up (arrowheads in E,F indicate rounding of the ventral-longitudinal muscles) and tend to form aggregates (arrows in E). (G,H) tsp^8R mutant embryos overexpressing TspA driven by stripe-gal4 (G) or by mef-2-gal4 (H) double-labeled with MHC (green) and with Tsp (red).