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Figure 3


Fig. 3. Inca expression in NC depends on Tfap2a. (A) Northern analysis of animal cap RNA from embryos injected with Chd, Chd+Wnt3a, or Chd+Wnt3a+dnTfap2a (Chd+Wnt+{Delta}AD) and probed for Xenopus Inca, with 18S rRNA as a loading control. Expression induced by Chd+Wnt is blocked by dnTfap2a. (B-G) Whole-mount in situ hybridizations showing zebrafish inca1 expression in wild type (B-D) and low mutants (E-G). B,E are lateral views and C,F dorsal views at the 10-somite stage. D,G are lateral views at 36 hpf. inca1 expression is reduced at 10 somites and 36 hpf. Numbers indicate presumptive pharyngeal arches. oe, olfactory epithelia.