Fig. 1. ems is expressed in one cluster of clonally related cells per hemisphere. Frontal views of adult brains. Anti-Ems labelling is magenta; the neuropile marker Nc82 is white; GFP-labelled wild-type MARCM clones are green. (A,B) z-projection of optical sections. Dashed line indicates border between central brain and optic lobes. (C) Single optical section showing ems expression in cells between antennal lobes and suboesophageal ganglion (SOG). (D,F,G) Individual wild-type clone shows co-localization of GFP with Ems in adult-specific cells. Single optical section (higher magnification of selected area, boxed) reveals that all GFP-labelled cells express ems, whereas a small subset of ems-expressing cells lack GFP (arrowheads in F). At a deeper focal plane, the same clone as in F extends a projection medially into the superior medial protocerebrum (arrows in D,G) and arborizations into the adjacent SOG neuropile (asterisk in D,G). (E) Three-dimensional model of D, illustrating position of MARCM clone in relation to major neuropile compartments such as the SOG, antennal lobes, mushroom bodies and superior medial protocerebrum. Scale bars: 50 µm in A-D; 5 µm in F,G. AL, antennal lobe; CB, central brain; MB, mushroom body; OL, optic lobe; SMP, superior medial protocerebrum; SOG, suboesophageal ganglion.