Fig. 5. Reduction of cell numbers in ems mutant EM clones in the late larval brain. (A) Average cell numbers of wild-type, ems mutant and rescued clones at late wandering larval stage (96 hours ALH) are indicated in bar graph (for genotypes see Materials and methods). Wild-type (B-D), mutant (E-G) and rescue (H-J) clones co-labelled with anti-Ems (magenta) and anti-ß-GAL (green in C,F) or GFP (green in I) and shown in z-projections. Note that in I a membrane-bound GFP marker results in weaker overlap with the nuclear anti-Ems signal compared with the nuclear anti-ß-GAL in C,F. Digital 3D models were generated to visualize clone size (white in D,G,J). (Ems-positive cells not co-labelled with clonal marker are shown in light magenta.) Neuroblast outlined with dots in confocal images and in green in the 3D models. Scale bars: 5 µm.