Fig. 7. Targeted misexpression of opa causes ectopic dpp^s-hc-lacZ expression. (A) dpp^s-hc-lacZ expression (green) in a wild-type disc. (B) ß-galactosidase expression (red) driven by the MS1096-Gal4. (C) Ectopic expression of dpp^s-hc-lacZ from MS1096-Gal4>Opa. Note boxed areas of ectopic expression from the dpp^s-hc-lacZ reporter on the medial side of the disc. The white arrow indicates the region of Opa expression where dpp^s-hc-lacZ is not expressed. Cytoplasmic ß-galactosidase expression (green) and Opa overexpression (nuclear) as detected by Opa antibody (red). (D,E) Higher magnification images of the areas boxed in C. (F) Cross section of area in E, showing that ß-galactosidase expression (green) and Opa antibody (red) are colocalized within the peripodial epithelium (arrow). (G) ß-galactosidase expression (red) driven by the c309-Gal4 driver. (H) Ectopic expression of dpp^s-hc-lacZ from c309-Gal4>Opa. A small area of ectopic ß-gal expression is boxed. (I) Higher magnification of the box in H. Nuclear Opa (red) and cytoplasmic ß-galactosidase expression (green) are colocalized. (J) Cross-section analysis of I. dpp^s-hc-lacZ and Opa expression are again limited to the peripodial epithelium (arrow). The nuclei of all discs are stained with DAPI (blue). The peripodial epithelium is oriented up in F and J, and lateral is to the left in all pictures.