Fig. 2. EGFR signalling activates IroRE²-lacZ. (A-B') Expression of lacZ (red) is activated in clones of cells (green) expressing activated forms of Ras (A,A') or Raf (B,B') (arrows) except in the wing pouch (arrowheads). Insets in B,B' show enlarged and enhanced pictures of the central wing pouch. Clones were induced at 48-72 (A,A') or 72-96 hours AEL (B,B'). (C,C') lacZ expression is lost (arrows) in clones of cells expressing a dominant negative form of Raf (green, induced at 24-48 hours AEL).