Fig. 3. Morphological analysis of Man1^GT/GT embryos. (A,B) Sagittal sections of wild-type (+/+) and Man1^GT/GT (GT/GT) E9.5 embryos stained with H&E. Left panels are low magnification views of sagittal sections. Right panels are high magnification views of frontal sections showing overall growth retardation and abnormal cardiac development in the Man1^GT/GT embryos. (C) Man1^GT/GT E9.5 embryo stained for lacZ expression with strong expression in the heart, amnion and allantois. (D) Sagittal section of an Man1^GT/GT E10.5 embryo stained for lacZ expression. Inset is at higher magnification. (E) Yolk-sac section of Man1^GT/GT E9.5 embryo shows disruption in the endodermal and mesodermal layers. (F) lacZ staining in cultured Man1^GT/GT MEFs, showing strong localization to the nuclear envelope. Scale bars: 1 mm in A-E; 50 µm in E,F. ne, neuroectoderm.