Fig. 5. oskar mRNA localization is affected at mid-oogenesis in rab6-null egg chambers. (A-D) Stage-9 egg chambers stained to reveal F-actin (phalloidin, red), Kin:ß-gal (green), Staufen (blue) and DNA (DAPI, white). (E-H) Co-detection of oskar mRNA (red) and Oskar protein (green), and DNA (DAPI; blue). In wild type, Kin:ß-gal and Staufen (A), and oskar mRNA and Oskar protein (E) colocalize in a sharp crescent at the oocyte posterior. All rab6^D23D egg chambers evaluated in this assay displayed a similarly affected actin cytoskeleton and the stereotypical pattern of eight- and four-nurse cell open syncytia (B-D). In nearly one third of these, localization of Kin:ß-gal and Staufen (B), and of oskar mRNA and Oskar protein (F), appears normal. In the remaining affected rab6^D23D egg chambers, Kin:ß-gal and Staufen/oskar are detected in blobs either partially (C,G) or completely (D,H) delocalized from the oocyte cortex. Oskar protein is detected at the posterior cortex only (G''), and not with unlocalized oskar mRNA (G'',H'').