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Figure 3


Fig. 3. Shroom3 is required for assembly of discrete arrays of parallel MTs in elongating neural epithelial cells. (A) High-magnification view of MTs in control hingepoint cells (green, anti-{alpha}-tubulin; red, propidium iodide, nuclei). (a') Schematic of a cell shown in A. Arrow indicates parallel MT arrays. (B) High-magnification view of MTs in dorsolateral hingepoint (DLHP) cells lacking Shroom3 function. (b') Schematic of a cell shown in B. (C) High-magnification view of control hingepoint cells (green, ZO-1; red, anti-{alpha}-tubulin). (D) High-magnification view of hingepoint cells lacking Shroom3 function. Shape changes are eliminated, but ZO-1 remains apically localized at cell-cell junctions.