Fig. 4. Shroom3 controls the distribution of -tubulin in neuroepithelial cells during neural tube closure. (A) High-magnification projection of a stack of optical sections showing elongating neural epithelial cells stained for -tubulin (white lines indicate cell outline). (a') -tubulin staining for cells shown in A. (B) Schematic of the closing neural tube indicating location of section shown in A (red box). (C) -tubulin staining in a projection of optical sections reveals centrosome-like foci at the apical surfaces of mature neuroepithelial cells. (c') Acetylated--tubulin staining reveals cell outlines in the ventral region of the closed Xenopus neural tube. (D) Schematic of the neural tube indicating location of section shown in C (red box). (E) Optical section showing -tubulin in an embryo unilaterally injected with Shroom3-MO (right side is injected, left side is control). (e') -tubulin staining for cells shown in E.