Fig. 5. Expression of Bruno and Lola in stwl mutant cells. (A,B) Bruno (Bru) expression (red) in stwl mutant GSCs (marked by the absence of GFP, green, and outlined) was similar to that of neighboring wild-type germ cells. (C-F) Bru expression (red) was uniform in bam mutant germaria (C,D) but was elevated in stwl bam ovaries (E,F) as differentiating germ cell clusters formed (arrows). Spectrosomes/fusomes (green) were labeled in C and E to show the degree of cyst development. (G,H). Germline clones of stwl mutant cells (marked by the absence of GFP, green, and outlined) contained higher levels of Lola (red) than neighboring cells.