Fig. 3. Cad6B and Snail2 have largely non-overlapping expression patterns in the trunk. Whole-mount in situ hybridizations (A,E) and immunohistochemistry (B-D,F-H) of 21- to 23-somite stage chick embryos for Snail2 (A-D) and Cad6B (E-H) expression. High levels of Snail2 mRNA and protein are detected in the dorsal neural tube as somites form, at axial levels that coincide with neural crest EMT. Conversely, Cad6B mRNA and protein are high in the caudal region of the embryo and decrease at more rostral regions at levels where neural crest EMT is occurring and migration is about to commence. nt, neural tube; psm, presomitic mesoderm; s, somite.