Fig. 2. Signals from the anterior-animal cells induce neural fate and suppress notochord fate. (A-J) Expression of a notochord-marker, Ci-Bra (A-E), and a neural marker, Ci-ETR (F-J), analysed at the 110-cell stage by in situ hybridisation in embryos and partial embryos derived from isolated cells, as indicated. (K,L) Histograms showing the average numbers of cells positive for Ci-Bra (magenta) and Ci-ETR (blue) in partial embryos as indicated on the left of the graphs. The data are expressed as mean±s.e.m. Significant differences compared to the results obtained by A4.1 isolation (for K) or by A6.2 or A6.4 isolation at the 24-cell stage (for L) were evaluated by the Student's t-test and indicated with asterisks (P<0.01). n, total number of samples analysed. Scale bars: 25 µm.