Fig. 2. The tombola region and analysis of predicted protein sequence. (A) tombola genomic region adapted from FlyBase. The tomb 5' UTR is 130-191 bp long; the 3' UTR is 71 bp long. Black boxes, coding sequence; grey boxes, UTRs; inverted triangles, insertion sites of P[EY]00456 (EY) and P[GS]12862 (GS). (B) Predicted sequence of Tomb protein. Thick underline, predicted nuclear localisation signal; bold, CXC region; light grey box, P[GS]12862 insertion site; thin underline, C-terminal conserved region. (C) Alignments of CXC domains and spacer from tomb; Drosophila mip120; human (hs) tesmin (tes) and tesmin-like (tesl); Ciona intestinalis (Ciona); Strongylocentrotus purpuratus (S. purp); C. elegans LIN-54; Arabidopsis (At) TSO1 and SOL2. Con(1) and Con(2) indicate amino acids in the first and second CXC domains: ^*, conserved Cys; =, residues conserved in both CXC domains; , residues conserved within CXC(1) or CXC(2), but which differ between the domains. The E(z) Cys-rich region is shown as an outgroup. (D) Alignment and predicted secondary structure (beneath) of the animal tesmin-family protein C-termini. Predictions of secondary structure are shown in the same order as the sequences (i.e. the first line is the Tomb secondary structure prediction). Black lines indicate high confidence helix predictions; the intervening region (grey line) has either no strong structural prediction (Tomb), or a strong coil prediction (Mip120, Hs-tes, Hs-tesl).