Fig. 7. Aly and Topi mislocalise in tomb testes. (A-L) Anti-Topi immunostaining (A,D,G,J, green) and DNA staining (B,E,H,K, red) in mature primary spermatocytes. In wild-type primary spermatocytes (A-C), Topi protein was predominantly chromatin associated. In achi/vis cells (G-I), Topi staining was distributed throughout the nucleus, but was brighter on chromatin, whereas the nuclear Topi staining in aly (D-F) and tomb (J-L) cells was less concentrated on chromatin. (M-R) Anti-Aly immunostaining (M,P, green) and DNA staining (N,Q, red) in mature primary spermatocytes. In wild type (M-O), Aly protein was nuclear and concentrated on chromatin. Aly protein was nuclear, but excluded from chromatin in tomb cells (P-R).