Fig. 8. Tomb nuclear localisation in late primary spermatocytes depends on comr, but not achi/vis. EGFP (A,B) and phase contrast (C,D) of achi/vis; Bam-Gal4-VP16, UAS-EGFP-Tomb (A,C) or comr; Bam-Gal4-VP16, UAS-EGFP-Tomb (B,D) whole testes and (insets) mature primary spermatocytes. Initially, the EGFP-Tomb localisations in achi/vis and comr are indistinguishable (A,B, arrows). Nuclear EGFP-Tomb was retained in achi/vis, but lost from comr mature spermatocytes (A,B, arrowheads). Arrested achi/vis spermatocytes had nuclear EGFP-Tomb (A, inset), whereas arrested comr spermatocytes had low levels of exclusively cytoplasmic fusion protein (B, inset).