Fig. 6. No obvious structural defects in cilia in Kupffer's vesicle or pronephric ducts of pkd2 morphant and mutant embryos. (A-D) Immunofluorescence performed with antibody against acetylated tubulin that allows for visualization of cilia. Cilia at Kupffer's vesicle in wild-type and pkd2 augMO embryos at 12 somites (A,B), and cilia in the pronephric ducts in wild type and in cup^tc321 mutants (C,D), are similar in shape and length. Wild-type Kupffer's vesicle cilia are 4.7±1.4 µm and pkd2 augMO Kupffer's vesicle cilia are 4.6±1.3 µm in length.