Fig. 7. Defects in ale1 ale2 and ale1 acr4 double mutants in the expression of protoderm-specific genes. (A-L) Accumulation of PDF1 (A-D), FDH (E,F,K,L) and ATML1 (G-J) transcripts, as visualized by in situ hybridization of the following embryos: wild type (A,E,G), ale1-1 (B), ale2-1 (C) and ale1-1 ale2-1 (D,F,H) on the Ler background; and wild type (I), acr4-1 (K) and ale1-1 acr4-1 (J,L) on the Ws background. (M-P) 5-day-old seedlings harboring the pPDF1::GFP construct. (M) A representative GFP-fluorescence image of a wild-type plant (inset) that expressed the pPDF1::GFP construct. (N) Magnified view of the central boxed region in M. (O,P) Gross morphology of an ale1 ale2 seedling with a disorganized leaf-like structure (O) and disorganized fluorescence due to GFP in the leaf-like organ (P). (P) Corresponds to the boxed area in O. The malformed young leaf exhibits a patchy pattern of fluorescence. Scale bars: 50 µm in A-L; 1 mm in M,O; 200 µm in N,P.