Fig. 3. CoIP and BiFC analysis for the interaction between DRN or DRNL and PHV. (A,B) Full-length PHV protein can precipitate fulllength DRN (A) or DRNL (B). Protein mixtures were precipitated via PHV-HA. The grid summarises the IP and Co-IP lanes and the antibodies used. Asterisks mark the co-eluted IgG light chain. (C-E) Cellular fluorescence of GFP-DRN (C), GFP-DRNL (D) or GFP-PHVs (E) in leek epidermal cells. (F,G) Bimolecular fluorescence complementation showing YFP expression observed with a GFP filter in leek epidermal cells following co-bombardment of full-length DRN and PHVs proteins (F) or full-length DRNL and PHVs proteins (G) fused to complementary YFP subdomains.