Fig. 3. Defects in migration of SCG cells in Creb-null mice. Parasagittal sections of E15.5 mouse embryos immunostained with TH antibody show the SCG normally shaped and located in proximity to the inner ear (asterisk) in control (A) and Creb^DBHCre; Crem^-/- mutants (B), but not in Creb^-/- mutants (F). The stellate ganglion is located in the thoracic region in the Creb^DBHCre; Crem^-/- mutant (D) and in the respective control littermate (C) as well as in the control littermate of Creb^-/- (E). However, in Creb^-/- it extends more rostrally (F). At E17.5 a similar pattern is found in Creb^-/- showing less sympathetic neurons in the area of the SCG (H, circled area) and more in the area of the stellate ganglion in comparison with control (G). Black arrowhead indicates the tubercle of the first rib, used as a positional reference to compare the position of the stellate ganglia. Scale bar: 300 µm in A-F; 600 µm in G,H.