Fig. 2. DjCHC-RNAi planarian could not regenerate the CNS. (A) Equal protein loadings of homogenates of 7-day-regenerated head region of either control or DjCHC-RNAi-treated planarians were subjected to SDS-PAGE using 7% gels, and western blots were probed with antibodies against the indicated protein. C, control; R, DjCHC-RNAi treated. (B) Dorsal view of the injected organisms (bright-field images). The left (control) and right (DjCHC-RNAi) panels show the 7-day regenerates of trunk pieces. The control planarian regenerated the tail and the head, as indicated by the dashed lines. The DjCHC- RNAi-treated planarian did not regenerate (arrowheads). a, anterior; p, posterior. (C) Defects of DjCHC-RNAi-treated planarians 7 days after amputation were detected in the CNS architecture, as revealed by immunostaining with anti-DjSYT and anti-DjGß. The dashed line indicates the border between the newly formed region and old stump region. Scale bars: 100 µm.