Fig. 3. No effects on the tail, pharynx, intestinal tract or muscles of DjCHC-RNAi-treated planarian. (A) Control and DjCHC-RNAi-treated planarian head piece at 7 days after amputation showing tail regeneration. Upper panels show DjAbd-Ba expression (blue) visualized using whole-mount in situ hybridization. Lower panels show VNCs of regenerated head pieces visualized with anti-DjSYT. White arrows, VNCs; black arrows, commissure neurons. VNCs of DjCHC-RNAi-treated planarians were perturbed. The dashed line indicates the border of the newly formed region and old stump region. (B) Pharynx regeneration of the head piece of the control and DjCHC-RNAi-treated planarian 7 days after amputation. The upper panels show pharynx-specific-gene DjMHC-A expression (blue) by whole-mount in situ hybridization. The lower panels show nerves of the pharynx visualized using anti-DjSYT. The dashed line indicates the pharynx. (C) Regeneration of the intestinal tract of a tail piece of a DjCHC-RNAi-treated planarian 7 days after amputation. Schematic drawings in A-C show the original expression patterns. (D) Body-wall musculature of a control and DjCHC-RNAi-treated planarian 7 days after amputation visualized with anti-DjMHC-B. Scale bars: 50 µm.