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Figure 7


Fig. 7. TUNEL assay of control and DjCHC-RNAi-treated R4HAC. (A-D) TUNEL-positive signals (green) at 2 DIV were visualized in DjCHC-RNAi-treated R4HAC. Antibody staining showed the expression of planarian 14-3-3{epsilon} or DjCHC as counterstaining (red), and Hoechst 33342 staining showed nuclei (blue). White arrows indicate neurite-extending TUNEL-negative cells. White arrowheads indicate neurite-less TUNEL-positive cells. Yellow arrows indicate the DjCHC-positive cells in DjCHC-RNAi-treated R4HAC. Scale bar: 10 µm. (E) Percentage (±s.e.m.) of TUNEL-positive cells out of total cells during culturing. (F,G) The percentage (±s.e.m.) of TUNEL-positive cells in the DjCHC-protein-expressing and DjCHC-protein-negative cell fractions at 3 DIV. ***P<0.01; NS: not significant.